The European Food Safety Authority (EFSA) has concluded that the food enzyme endo-1,3(4)-β-glucanase from the non-genetically modified Rasamsonia composticola strain 427-FS does not pose a safety concern under revised intended conditions of use, including two additional food manufacturing processes. The scientific opinion, adopted on 24 June 2026 and published on 10 July 2026, updates a previous safety evaluation from December 2022 that had already deemed the enzyme safe for six processes. The extension covers production of plant extracts and plant-based analogues of milk and milk products, bringing the total to eight processes.
The dietary exposure to the food enzyme–Total Organic Solids (TOS) was estimated for the six processes where TOS remain in the final food, reaching up to 1.368 mg TOS/kg body weight per day in European populations. Combined with the previously reported no observed adverse effect level of 866 mg TOS/kg bw per day, the margin of exposure is at least 633, which EFSA considers sufficient. The enzyme is inactivated in most processes, though residual activity may remain in baked products.
The applicant, Kerry Ingredients & Flavours Ltd., submitted the extension request on 29 November 2024. EFSA's assessment follows the EU's regulatory framework for food enzymes under Regulation (EC) No 1332/2008, which requires safety evaluation before inclusion in the Union list. The opinion will inform the European Commission's decision on authorisation.